Upon registration with us, each customer will be assigned a barcode label with a registration number for all future identification. We will also provide each customer with a collection kit for their doctor's use.
After the baby is delivered, your doctor will collect the cord blood remaining in the umbilical vein and placenta using the sterilized closed-system collection bag set we provided. The collection process is user-friendly and painless.
3 Transport to Laboratory
Within 12 hours after notification from the customer, our dedicated transport staff will arrive the hospital with a temperature-controlled cell safe to collect the kit.
4 Verification of Information and Sample
At the laboratory, our professional technician will verify the identity information and test the blood specimens for infectious diseases and blood grouping. Before processing, a small cord blood sample will be retrieved for stipulated tests.
5 Automated Processing
We will remove red blood cells and plasma from the cord blood sample to obtain cord blood stem cells. This process will be performed by an advance automated cell recovery system which is functionally closed and sterile, ensuring effective contamination control and efficient recovery of the mononuclear cells (MNCs) from cord blood. After this process a small concentrated cord blood aliquot is prepared for stipulated tests.
6 Controlled-Rate Freezing and Storage
After processing, we will add cryoprotectant to the processed cord blood unit and it will be ready for storage. The unit will undergo a slow freezing process at a controlled rate until it reaches -90'C before it is transferred to a vapour phase liquid nitrogen tank for long-term storage at -185 to -190'C.
7 On-going Quality Assurance
We have put in place a biannual quality assurance program to ensure the good quality of cryopreserved cord blood units in each liquid nitrogen tank. Terminated cryopreserved units will be retrieved and thawed for quality tests including cell viability and cord blood stem cell count. Cord blood stem cell activities are also analyzed by performing colony formation unit (CFU) assay.
Cord / Cord Blood Collection Box
Upon registration with CRYOLIFE, each customer will be assigned a cord blood collection box (BB box) with a barcode label for their doctor's use.
The box contains tools required for cord blood collection: sterilized triple blood bag (with anticoagulant), vacuum tube for maternal blood collection, sterilization tools and other necessary materials. A client’s reminder and documents regarding cord blood collection are attached.
The BB box should be kept in cool dry place. Clients are obligated to notify her O&G physicians about the decision of cord blood banking. Please be reminded to take the BB box along to hospital for delivery. Pass the box to midwife or nurse and the professional medical staff will arrange cord blood banking with you.
Cord blood & Cord Collection Procedures
Cord Blood Collection Procedure
Cord Collection Procedure
Dedicated Transportation Team
After baby was born and cord blood is collected, client should call CRYOLIFE 24-hour hotline at 2110 2121. We will send our dedicated transport staff to pick up the BB box.
We promise to arrive at the hospital for BB box pick up within 12 hours after instruction from customers. (24 hours for Macau customers.)
Processing & Storage
Best in Class Technologies
In 2008, CRYOLIFE was the first in Hong Kong to migrate to automated processing with the adoption of the AXP Autoxpress™ Platform which achieved a higher recovery rate than manual processing. With the control rate freezing and MVE vapor phase liquid nitrogen storage tanks, we provide the best in class technologies in the market.
AXP Processing Bag Set lot no. is now marked on your Test and Storage Report
Since then, CRYOLIFE process cord blood sample with AXP processing system. We promises “What you paid is what you get”!CRYOLIFE will NOT just claim to use AXP but process manually in behind. To guarantee our service commitment, AXP Processing Bag Set lot no. is now marked on the test and storage report. Clients can understand what they pay for.
Test and Storage Report Sample (a part):
Please click here to distinguish between AXP AutoXpress™ Platform and Manual Processing
|Comparison between AXP and other processing method|
|AXP™ Automated System||Sepax™ Automated System||Manual Processing|
|Processing Time||~40min.||~40min.||~2 - 4hr.|
|TNC Recovery Rate||>97%*||81.0%#||80 - 90%^|
Remarks: The highest recovery rate by CRYOLIFE with AXP™ automated system is 99%
source： * GE Healthcare
#actual data collected in a test run by CRYOLIFE in 2007
^statistic figures by CRYOLIFE's past manual processing data
Slow Freezing Process
The cord blood sample after processing need to undergo a slow freezing process at a controlled rate until it reaches -90'C before it is transferred to a vapour phase liquid nitrogen tank for long-term storage at -185 to -190'C.
|Controlled Rate Freezer|
|Processing procedure||slow freezing by a rate of 1℃ to 2℃ per min.until -90℃|
Freezing with Sample Probe to monitor the actual freezing situation
Full computer control. In response to th releasing of latent heat, the system will adjust the freezing rate to protect the cells
More than 10 samples can be processed at one time, enhances processing efficiency
Emergency Plan and Safety Compliance
In 2008, CRYOLIFE devoted $5 million to upgrade the laboratory for cord blood processing and storage, and to improve the room safety, while over $1 million was spent on vacuum steel pipes installation.
Quality steel pipes manufactured in German.
Ensure stable nitrogen supply from nitrogen source to MVE storage tank.
Minimize nitrogen leakage to protect safety of technicians and storage room.
|Planer Assure24Seven Monitoring System + 24-hr Surveillance Alarm System||
Specialized for monitoring temperature of apparatus in laboratory
24-hr computerized system, record the condition of the storage tanks
All management team, laboratory team and transport team are equipped with Blackberry, related management staff will be notified and take corresponding action if storage tanks in abnormal situation
Round the clock surveillance to protect the corb blood smaples are in stable storage condition
|Backup Power Supply|
|Backup Battery + UPS Uninterruptible power supply system||
lasting for 72 hours in emergency and remain normal operation for each storage tank
UPS retain a steady electric current
centrifuge can run normally even facing an unexpected power failure, no cord blood processing will be interrupted
execute contingency plan with good flexibility
|Fire and Buliding Safety Compliance, Location|
|Storage rooms are tiled with certified fire safe building materials + Full compliance with the fire and building safety regulations with||
sufficient anti-fire capability, management team have enough time for taking contingency action
|Contingency plan conform to AABB accreditation||
comprehensive contingency plan to cope with disaster like earthquake, tornado, fire and etc
in emergencies, management team have sufficient ability to protect the storage tanks and cord blood sample
|Located at Delta House, Siu Lek Yuen, Shatin||
located in transport hub of Shatin, cord blood arrive the laboratory in many ways
to minimize the change of storage condition, our management had made a responsible decision that not to relocate our facilities even though we have been invited by the Hong Kong Science & Tehcnology Parks
Test and Storage Report
After the cord blood sample is stored, a detail test and storage report will be sent for client’s future reference. We promise the test and storage report will be sent to parents 2 months after baby was born.
|Cord Blood Storage||Blood Test|
Service Commitment and Refund Policy
CRYOLIFE is obligated to serve the community’s needs in cord blood storage by providing the best in class collection, processing and storage service. With the following service commitment, we guarantee a quality cord blood banking service to clients.
All fees paid will be refunded to customer for the above situation. Also, if the cord blood sample is released for clinical purpose as per client’s request, the remaining storage fee will be refunded.
Using the cord blood / cord
CRYOshipper is a professional container dedicated to cryopreserved stem cell products transport. It ensures the cryopreserved unit will be kept at 150℃ for as long as 60 hours in transportation. The transport process will be monitored by an electronic device.
CRYOshipper has conformed with the Code of Federal Regulation (CFR) and is suitable for cord blood sample delivery. It has also passed IATA and able to be couriered by air.
To be well prepared of the life saving mission, our laboratory team conducts regular rehearsal for preparation of delivery by CRYOshipper.
Umbilical Cord Blood Net Volume Report
Cord blood cell count is directly proportional to the volume of cord blood collected. The higher the cell count of cord blood, the more beneficial to the clinical application of cord blood stem cells.
The doctors listed follow paid extra effort in cord blood collection, which is crucial to a person's life. Let's give our big hand to them.
June 2016 Doctors' Performance: Top 5 Highest UCB Net Volume Collected
|Rank||Doctor||Highest Net UCB Volume Collected (ml)|
|1||Hon Hing Cheung Edmund
|2||Lam Sze Wing Helena||90.7|
|3||Liauw Linna Angeline
|4||Ho Wing Chiu
Chan Suk Man Anne
*Average UCB volume collected in June 2016: 59.1 ml
To view Past UCB Volume Reports